Since 2020, the Washington Nationals have lost the most games by a team, with 425 losses.

Interpreted as:

most regular-season losses by an MLB team since 2020

	TEAM	L	W	L%	G	W%	AB	R	H	2B	3B	HR	RBI	BB	HBP	SO	SB	CS	PA	TB	XBH	SH	SF	IBB	GIDP	AVG	OBP	SLG	OPS
1	Nationals	425	289	.595	714	.405	23,880	3,001	6,008	1,193	96	679	2,852	2,102	329	5,411	520	173	26,588	9,430	1,968	110	167	88	570	.252	.319	.395	.714
2	Rockies	424	289	.595	713	.405	24,118	3,126	6,001	1,228	141	739	2,993	2,016	243	6,457	326	111	26,654	9,728	2,108	95	182	74	530	.249	.311	.403	.714
3	Pirates	419	296	.586	715	.414	23,716	2,795	5,494	1,065	119	664	2,658	2,241	236	6,382	407	135	26,467	8,789	1,848	98	176	69	428	.232	.302	.371	.673
4	Athletics	412	303	.576	715	.424	23,593	2,836	5,366	1,082	85	785	2,704	2,227	335	6,310	439	103	26,413	8,973	1,952	89	169	52	490	.227	.301	.380	.681
5	Royals	405	309	.567	714	.433	23,908	3,008	5,873	1,129	153	706	2,882	1,910	249	5,679	579	168	26,384	9,426	1,988	98	219	54	420	.246	.306	.394	.700
6	White Sox	401	313	.562	714	.438	24,091	2,959	5,878	1,136	59	748	2,830	1,940	270	6,100	314	94	26,521	9,376	1,943	75	145	47	550	.244	.306	.389	.695
7	Angels	398	316	.557	714	.443	23,923	3,038	5,710	1,062	105	865	2,904	2,168	307	6,413	386	143	26,633	9,577	2,032	82	153	104	492	.239	.308	.400	.709
8	Marlins	398	317	.557	715	.443	23,939	2,804	5,786	1,069	95	683	2,665	1,934	268	6,280	496	138	26,387	9,094	1,847	90	156	79	536	.242	.304	.380	.684
9	Rangers	392	323	.548	715	.452	24,100	3,138	5,795	1,083	86	843	2,987	2,161	240	6,119	463	128	26,710	9,579	2,012	44	165	62	431	.240	.307	.397	.705
10	Diamondbacks	386	328	.541	714	.459	23,993	3,320	5,876	1,232	148	760	3,146	2,387	295	5,820	458	109	27,032	9,684	2,140	136	221	72	469	.245	.318	.404	.722
11	Tigers	380	332	.534	712	.466	23,782	2,873	5,624	1,047	155	684	2,764	2,025	222	6,492	319	103	26,256	9,033	1,886	45	182	40	463	.236	.300	.380	.680
12	Reds	377	337	.528	714	.472	23,662	3,183	5,649	1,136	100	847	3,045	2,307	412	6,396	524	160	26,622	9,526	2,083	84	157	47	491	.239	.315	.403	.718
13	Cubs	367	350	.512	717	.488	23,923	3,242	5,771	1,114	125	823	3,082	2,397	378	6,444	515	148	26,965	9,604	2,062	92	175	73	490	.241	.318	.401	.719
14	Orioles	360	355	.503	715	.497	24,172	3,236	5,955	1,224	108	872	3,109	2,112	288	6,151	384	119	26,820	10,011	2,204	68	180	56	424	.246	.312	.414	.726
15	Red Sox	359	356	.502	715	.498	24,496	3,406	6,309	1,461	92	838	3,249	2,181	290	6,321	388	120	27,188	10,468	2,391	44	177	99	512	.258	.323	.427	.751
16	Twins	356	358	.499	714	.501	24,019	3,234	5,859	1,203	85	918	3,083	2,311	357	6,289	259	82	26,909	9,986	2,206	41	181	65	530	.244	.317	.416	.733
17	Mets	343	371	.480	714	.520	23,792	3,202	5,886	1,117	88	862	3,066	2,261	454	5,866	363	96	26,746	9,765	2,067	84	155	80	515	.247	.323	.410	.733
18	Cardinals	341	371	.479	712	.521	23,831	3,151	5,902	1,155	83	831	3,024	2,296	327	5,734	400	117	26,725	9,716	2,069	80	191	76	466	.248	.320	.408	.728
19	Guardians	336	377	.471	713	.529	23,866	3,053	5,811	1,165	106	705	2,894	2,097	314	5,413	552	139	26,553	9,303	1,976	80	196	129	484	.243	.311	.390	.700
20	Giants	333	381	.466	714	.534	23,943	3,216	5,767	1,164	100	866	3,083	2,422	321	6,430	280	76	26,944	9,729	2,130	74	184	101	511	.241	.317	.406	.723
21	Mariners	332	383	.464	715	.536	23,693	3,093	5,443	1,065	61	858	2,967	2,486	422	6,728	461	127	26,807	9,204	1,984	40	166	57	450	.230	.312	.388	.700
22	Blue Jays	332	383	.464	715	.536	24,252	3,372	6,229	1,285	74	899	3,234	2,281	269	5,552	354	113	27,011	10,359	2,258	46	163	63	546	.257	.326	.427	.753
23	Padres	328	387	.459	715	.541	23,969	3,307	5,966	1,196	83	829	3,167	2,501	268	5,558	486	135	27,040	9,815	2,108	117	185	108	525	.249	.324	.409	.734
24	Phillies	327	387	.458	714	.542	24,095	3,400	6,085	1,195	117	912	3,259	2,350	279	6,142	511	113	26,991	10,250	2,224	88	179	112	481	.253	.324	.425	.749
25	Brewers	317	397	.444	714	.556	23,768	3,238	5,647	1,103	89	836	3,099	2,593	310	6,450	544	132	26,885	9,436	2,028	58	156	95	515	.238	.319	.397	.716