Since 2020, the Washington Nationals have lost the most games by a team, with 420 losses.

Interpreted as:

most regular-season losses by an MLB team since 2020

	TEAM	L	W	L%	G	W%	AB	R	H	2B	3B	HR	RBI	BB	HBP	SO	SB	CS	PA	TB	XBH	SH	SF	IBB	GIDP	AVG	OBP	SLG	OPS
1	Nationals	420	288	.593	708	.407	23,683	2,980	5,966	1,182	96	670	2,831	2,086	327	5,344	514	171	26,372	9,350	1,948	109	167	88	568	.252	.319	.395	.714
2	Rockies	419	288	.593	707	.407	23,921	3,115	5,961	1,221	140	736	2,982	2,005	239	6,389	324	108	26,441	9,670	2,097	95	181	74	528	.249	.311	.404	.716
3	Pirates	414	294	.585	708	.415	23,482	2,776	5,448	1,060	118	660	2,640	2,213	232	6,316	388	133	26,199	8,724	1,838	98	174	67	424	.232	.302	.372	.674
4	Athletics	407	301	.575	708	.425	23,360	2,813	5,315	1,076	85	774	2,681	2,207	334	6,260	439	103	26,158	8,883	1,935	88	169	52	482	.228	.301	.380	.682
5	Royals	401	307	.566	708	.434	23,701	2,985	5,829	1,117	152	702	2,859	1,893	244	5,629	574	165	26,152	9,356	1,971	96	218	52	420	.246	.306	.395	.700
6	White Sox	397	311	.561	708	.439	23,899	2,936	5,837	1,131	59	739	2,807	1,925	270	6,056	311	93	26,312	9,303	1,929	75	143	47	548	.244	.306	.389	.695
7	Angels	396	312	.559	708	.441	23,726	3,014	5,671	1,056	103	861	2,880	2,152	303	6,363	382	142	26,411	9,516	2,020	81	149	102	484	.239	.309	.401	.710
8	Marlins	395	313	.558	708	.442	23,697	2,775	5,727	1,057	94	678	2,639	1,910	265	6,216	490	136	26,112	9,006	1,829	86	154	76	530	.242	.304	.380	.684
9	Rangers	390	318	.551	708	.449	23,886	3,120	5,754	1,075	86	836	2,969	2,145	237	6,075	459	128	26,476	9,509	1,997	44	164	62	427	.241	.308	.398	.706
10	Diamondbacks	384	324	.542	708	.458	23,795	3,282	5,822	1,216	148	752	3,108	2,358	292	5,779	454	108	26,796	9,590	2,116	134	217	71	466	.245	.318	.403	.721
11	Tigers	376	330	.533	706	.467	23,567	2,846	5,567	1,038	152	678	2,738	2,001	222	6,428	315	102	26,015	8,943	1,868	45	180	39	460	.236	.300	.379	.679
12	Reds	373	335	.527	708	.473	23,469	3,159	5,607	1,131	100	840	3,021	2,296	409	6,348	520	159	26,415	9,458	2,071	84	157	47	487	.239	.316	.403	.719
13	Cubs	363	345	.513	708	.487	23,594	3,182	5,687	1,094	124	809	3,022	2,354	375	6,365	504	148	26,587	9,456	2,027	92	172	72	489	.241	.318	.401	.718
14	Orioles	356	352	.503	708	.497	23,937	3,200	5,890	1,214	108	861	3,074	2,092	284	6,087	380	117	26,557	9,903	2,183	68	176	56	417	.246	.312	.414	.726
15	Red Sox	355	353	.501	708	.499	24,256	3,379	6,254	1,450	91	831	3,223	2,156	289	6,246	379	120	26,921	10,379	2,372	44	176	98	510	.258	.324	.428	.752
16	Twins	352	356	.497	708	.503	23,823	3,214	5,823	1,194	85	913	3,063	2,296	354	6,246	257	80	26,695	9,926	2,192	41	181	65	527	.244	.318	.417	.735
17	Mets	340	368	.480	708	.520	23,595	3,179	5,849	1,106	88	855	3,044	2,241	450	5,820	360	95	26,524	9,696	2,049	84	154	80	508	.248	.323	.411	.734
18	Cardinals	339	367	.480	706	.520	23,616	3,109	5,838	1,145	83	820	2,984	2,268	325	5,688	395	115	26,477	9,609	2,048	79	189	73	462	.247	.319	.407	.726
19	Giants	332	376	.469	708	.531	23,745	3,186	5,727	1,155	100	856	3,053	2,404	319	6,366	274	76	26,723	9,650	2,111	73	182	101	508	.241	.317	.406	.723
20	Guardians	332	375	.470	707	.530	23,672	3,033	5,767	1,156	105	698	2,874	2,079	311	5,364	552	136	26,332	9,227	1,959	79	191	128	479	.244	.311	.390	.700
21	Blue Jays	330	378	.466	708	.534	24,026	3,340	6,164	1,268	74	894	3,205	2,259	265	5,504	352	111	26,753	10,262	2,236	44	159	63	542	.257	.325	.427	.752
22	Padres	328	380	.463	708	.537	23,751	3,271	5,901	1,183	83	823	3,134	2,475	267	5,518	471	134	26,789	9,719	2,089	115	181	106	520	.248	.324	.409	.733
23	Mariners	328	380	.463	708	.537	23,489	3,075	5,408	1,060	60	851	2,950	2,457	418	6,662	455	125	26,567	9,141	1,971	39	164	57	448	.230	.312	.389	.701
24	Phillies	326	382	.460	708	.540	23,885	3,367	6,021	1,183	116	903	3,229	2,325	278	6,096	506	111	26,754	10,145	2,202	87	179	111	476	.252	.323	.425	.748
25	Brewers	313	395	.442	708	.558	23,557	3,215	5,600	1,095	89	830	3,078	2,572	307	6,382	539	132	26,649	9,363	2,014	58	155	93	513	.238	.319	.397	.716